Description
Number of Tests Included:
Single blood test detecting antibodies bound to RBCs.
What is a Direct Coombs Test?
A test that checks for antibodies stuck directly on the patient’s red blood cells. These antibodies cause RBC destruction, leading to anemia.
Purpose of the Test:
Diagnose autoimmune hemolytic anemia (AIHA)
Detect transfusion reactions
Identify hemolytic disease of the newborn (HDN)
Evaluate unexplained hemolysis
Assess drug-induced hemolytic anemia
Who Should Get This Test?
Individuals with sudden anemia or jaundice
Patients with suspected autoimmune disorders
Newborns with jaundice or suspected blood group incompatibility
Individuals after recent blood transfusion
Patients with enlarged spleen or recurrent hemolytic episodes
Components of the Test:
Detection of antibodies (IgG) bound to RBCs
Detection of complement (C3) — depending on lab
Understanding the Test Report:
Positive DCT → Antibodies/complement present on RBCs; suggests autoimmune or immune-mediated hemolysis
Negative DCT → No significant RBC-bound antibodies detected
Possible causes of Positive DCT:
Autoimmune hemolytic anemia
Hemolytic disease of newborn (Rh/ABO incompatibility)
Transfusion reaction
Certain medications (drug-induced hemolysis)
Infections or immune disorders
Preparation and Procedure:
Routine blood sample
No special preparation required
How is the Test Done?
The patient’s RBCs are washed and mixed with anti-human globulin (Coombs reagent). If antibodies are present on the RBC surface, agglutination occurs.
Best Time for Test:
Anytime symptoms or risk factors are present.
Booking and Checking Reports:
You can book the Direct Coombs Test via SDL, choose home blood sample collection, and access your digital report online.
